EQUIPMENT
– CELLULAr imaging

Confocal Zeiss LSM980 Airyscan 4Y

​Today’s applications require ever higher resolution, sensitivity and speed. So to meet this need we have equipped ourselves with an LSM880 Fast Airyscan (Zeiss).

This technology is more resolutive than confocal while having a better signal/noise and with more speed thanks to the Fast module.

The microscope on the platform is an Axio Observer Z1 motorised inverted microscope.

Four excitation sources are available:

• Diode (UV): 405nm
• Argon Laser: 458, 488 et 514nm
• Diode Laser (DPSS): 561nm
• Hélium Néon Laser: 633nm

The objectives present are:

• Plan-Apochromat   10x / 0,45 DICII
• Plan-Apochromat   20x / 0,8 DIC II
• Plan-Apochromat   40x / 1.3 oil DIC II
• Plan-Apochromat   63x / 1,4 oil DIC III 

Type of acquisition:

Zeiss Zen Blue software
– multi-modal, XYZ, time, multi-position and mosaic confocal imaging
– ‘super-resolution’ imaging
– live and ‘super-resolution’ imaging with the AiryScan Multiplex 4Y.
– Spectral module
– FRAP module

The instrument features full environmental control of temperature and CO2, as well as a motorised stage for multi-dimensional acquisitions on live cells and mosaic image acquisition.
A piezo stage is mounted on the microscope for fast, accurate z-stacks.

The Airyscan module improves resolution by a factor of 2x. Maximum resolution is 120 nm lateral (XY) and 350 nm axial (Z).

The AiryScan Multiplex 4Y considerably accelerates acquisition speed by scanning 4 lines simultaneously.