Team
Lorenzo Scipioni

OncoBreast​ :

Advanced microscopy for the study of chemoresistance and heterogeneity in breast cancer

The specificities

of our research axis

Cells, like people, possess tiny “organs”, called organelles, with specific roles that are fundamental to sustain a cell’s function and determine its suitability for specific tasks. Organelle properties vary enormously between cell types (e.g. non-tumorigenic and cancerous cells), and they can be used to “diagnose” a cell’s physiological state and how a cell can react to external stimuli, such as a chemotherapy treatment.

Using ESPRESSO (Environmental Sensors Phenotyping RElayed by Subcellular Structures and Organelles), a technique we developed that combines advanced microscopy, chemical tools and computer science, we are able to follow the physiological state of each individual cancer cell for extended periods of time, precisely characterizing the changes that drive the generation of chemoresistant populations.

Our overarching goal is to further develop this groundbreaking technology and apply to the study of triple-negative breast cancer, the most aggressive and resistant to therapy form of breast cancer. In this context, we plan on using ESPRESSO as a diagnostics and prognostics tool to improve triple-negative breast cancer classification, early relapse detection and treatment selection.

Team Oncobreast combines knowledge and expertise from physics, optical engineering, computer science, biology and medicine, to unite forces in our fight against breast cancer.

Breast cancer

Chemoresistance

Relapse

Triple negative

Microscopy

Hyperspectral

Single-Cell

Spatiotemporal

Spatial proteomics

Organelles

Machine learning

Neural networks

RESEARCH
PROJECTS

THE TEAM’S
FOCUS

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SCIENTIFIC PRODUCTIONS

PUBLICATIONS 2025
PUBLICATIONS 2024
Tedeschi, Giulia, Mariana X. Navarro, Lorenzo Scipioni, Tanvi K. Sondhi, Jennifer A. Prescher, and Michelle A. Digman. “Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis.” Chemical & Biomedical Imaging 2, no. 11 (November 25, 2024): 765–74. https://doi.org/10.1021/cbmi.4c00049.
Di Bona, Melody, Yanyang Chen, Albert S. Agustinus, Alice Mazzagatti, Mercedes A. Duran, Matthew Deyell, Daniel Bronder, et al. “Micronuclear Collapse from Oxidative Damage.” Science (New York, N.Y.) 385, no. 6712 (August 30, 2024): eadj8691. https://doi.org/10.1126/science.adj8691.
Carretta, Annalisa, Aldo Moscardini, Giovanni Signore, Doriana Debellis, Federico Catalano, Roberto Marotta, Valentina Palmieri, et al. “The Supramolecular Processing of Liposomal Doxorubicin Hinders Its Therapeutic Efficacy in Cells.” Molecular Therapy: Oncology 32, no. 3 (2024): 200836. https://doi.org/10.1016/j.omton.2024.200836.
Sakthivel, Pooja S., Lorenzo Scipioni, Josh Karam, Zahara Keulen, Mathew Blurton‐Jones, Enrico Gratton, and Aileen J. Anderson. “Organelle Phenotyping and Multi‐dimensional Microscopy Identify C1q as a Novel Regulator of Microglial Function.” Journal of Neurochemistry 168, no. 9 (2024): 3095–3107. https://doi.org/10.1111/jnc.16173.
Fazel, Mohamadreza, Lorenzo Scipioni, Enrico Gratton, Michelle A. Digman, and Steve Pressé. “Highly Multiplexed Spectral-FLIM via Physics-Informed Bayesian Analysis.” Biophysical Journal 123, no. 3 (2024): 432a–33. https://doi.org/10.1016/j.bpj.2023.11.2622.
Park, Sohyeon, Xiaorong Wang, Yajin Mo, Sicheng Zhang, Xiangpeng Li, Katie C. Fong, Clinton Yu, et al. “Proximity Labeling Expansion Microscopy (PL-ExM) Evaluates Interactome Labeling Techniques.” Journal of Materials Chemistry B 12, no. 34 (2024): 8335–48. https://doi.org/10.1039/D4TB00516C.
Torrey, Zachary R., Lila P. Halbers, Lorenzo Scipioni, Giulia Tedeschi, Michelle A. Digman, and Jennifer A. Prescher. “A Versatile Bioluminescent Probe with Tunable Color.” RSC Chemical Biology 5, no. 11 (2024): 1097–1103. https://doi.org/10.1039/D4CB00101J.

TEAM MEMBERS

Francesco Fersini
Chercheur Post-Doctorant / Post-Doc researcher
New
Giulia Tedeschi
Ingénieur de laboratoire / laboratory engineer

PARTNERSHIPS & FUNDING

 

Centre de Recherches en Cancérologie de Toulouse

Toulouse Cancer Research Center (Oncopole)

Toulouse - FR

Contact us

+33 5 82 74 15 75

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