EQUIPMENT
– geNomics
& TRANSCRIPTOMIcs
Single cell Chromium X
Isolation of single cells for gene expression analysis by NGS sequencing (10X Genomics)
This technology enables the capture of 500 to 10,000 cells/fresh, fixed or FFPE sample and the analysis of their individual gene expression.
This approach makes it possible to account for cellular heterogeneity. To better identify cell sub-populations or monitor the expression of a surface protein of interest, it is also possible to combine the measurement of individual gene expression with the identification of cell surface proteins using the CITE-Seq method (antibody/antigen labelling coupled to a nucleic acid).
The principle of the technology is based on the encapsulation of a cell in a droplet and the identification of its nucleic acids by a specific individual barcode.
Other applications are available: VDJ, ATAC-seq, Multiome (Expression + ATAC-seq), CRISPR screening, etc.
Access to this technology is by project service and specific quotation.
Toulouse Cancer Research Center (Oncopole)
Toulouse - FR
CRCT-Oncopole Follow 1,433 1,279
Compte officiel du Centre de Recherches en Cancérologie de Toulouse
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🇬🇧Cytidine deaminase-dependent mitochondrial biogenesis as a potential vulnerability in pancreatic cancer cells. ImPact team @CordelierPierre article from @crctoncopole published in @CommsBio
➡️https://www.crct-inserm.fr/en/targeting-the-energy-production-process-in-pancreatic-cancer-cells-which-relies-on-an-enzyme-called-cytidine-deaminase-could-make-these-cells-more-vulnerable/
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