Cytometry & Cell Sorting
EXPERTISE
A DEDICATED PLATFORM…
The Cytometry and cell sorting platform of the Technology Centre provides expertise to the regional academic and private scientific community. The service is equipped with 4 analysers and 2 cell sorters.
The platform operates under specific internal regulations, which are integrated into the internal regulations of the Technology Centre and validated by the CRCT Laboratory Council.
The Cytometry and Cell Sorting Department is located in the L2 containment area of the Technology Centre, on the 1st floor of the CRCT’s A building.
OUR MISSIONS…
Cell analysis and sorting for research teams of public or private organisations
Advice and assistance to users for data acquisition, analysis and interpretation
Training of users to achieve autonomy
Design and development of collaborative research projects
Formatting and communication of analysis results
AVAILABLE DEVICES
ANALYzers
Cytometers are freely available, depending on their availability, to users who have undergone compulsory training by the platform staff.
For other users, technical assistance is available by appointment.
MACSQuant 10 (Miltenyi Biotec)
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- 3 lasers (405nm, 488nm et 635nm)
- 8 fluorescence parameters
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MACSQuant VYB (Miltenyi Biotec)
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- 3 lasers (405nm, 488nm et 561nm)
- 8 fluorescence parameters
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AURORA (CYTEK)
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- 5 lasers (355nm, 405nm, 488nm, 561nm et 640nm)
- 30 fluorescence parameters
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LSRII (BD Biosciences)
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- 3 lasers (405nm, 488nm et 635nm)
- 11 fluorescence parameters
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FortessaX20 (BD Biosciences)
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- 5 lasers (355nm, 405nm, 488nm, 561nm et 635nm)
- 18 fluorescence parameters
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CELL SORTERS
Cell sorters allow the isolation and recovery of one or more cell sub-populations according to criteria of size, granulosity (cell content) and fluorescence (receptor, genetic transformation, cell phase, etc.).
The recovered cells can then be analysed biochemically or put back into culture.
FACS Melody (BD Biosciences)
- 3 lasers (405nm, 488nm et 561nm)
- 8 fluorescence parameters
- Under PSM
- Isolate or enrich up to 2 different cell subpopulations
- Cell cloning (96 or 384 well plates)
- Up to 10000 events per second
FACS Aria Fusion (BD Biosciences)
- 4 lasers (355nm, 405nm, 488nm et 640nm),
- 18 fluorescence parameters
- Under PSM
- Isolate or enrich up to 4 different cell subpopulations
- Cell cloning (96 or 384 well plates)
- Up to 30 000 events per second
INTERNAL REGULATIONS
All new users must have read the rules and regulations of the Cytometry Platform and returned the information sheet.
MEMBERS OF THE PLATFORM
Manon Farcé
Toulouse Cancer Research Center (Oncopole)
Toulouse - FR
CRCT-Oncopole Follow 1,433 1,279
Compte officiel du Centre de Recherches en Cancérologie de Toulouse
;
🇬🇧Cytidine deaminase-dependent mitochondrial biogenesis as a potential vulnerability in pancreatic cancer cells. ImPact team @CordelierPierre article from @crctoncopole published in @CommsBio
➡️https://www.crct-inserm.fr/en/targeting-the-energy-production-process-in-pancreatic-cancer-cells-which-relies-on-an-enzyme-called-cytidine-deaminase-could-make-these-cells-more-vulnerable/
Contact us
+33 5 82 74 15 75
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